LOCUS       Exported                5000 bp DNA     circular SYN 15-JUL-2025
DEFINITION  Shuttle vector pSET2 DNA, complete sequence.
ACCESSION   AB042430
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Takamatsu D, Osaki M, Sekizaki T.
  TITLE     Construction and characterization of Streptococcus suis-Escherichia 
            coli shuttle cloning vectors
  JOURNAL   Plasmid 45 (2), 101-113 (2001)
  PUBMED    11322824
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Takamatsu D, Osaki M, Sekizaki T.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2000) Daisuke Takamatsu, National Institute of 
            Animal Health, Laboratory of Molecular Bacteriology; Kannondai 
            3-1-1, Tukuba, Ibaraki 305-0856, Japan 
            (E-mail:p1013dt@niah.affrc.go.jp, Tel:81-298-38-7743, 
            Fax:81-298-38-7743)
REFERENCE   3  (bases 1 to 5000)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5000)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5000)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2001"; volume: "45"; issue: "2"; pages: "101-113"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-MAY-2000) Daisuke Takamatsu, National Institute of Animal
            Health, Laboratory of Molecular Bacteriology"; pages: 
            "81-298-38-7743"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5000
                     /lab_host="Streptococcus suis, Escherichia coli"
                     /mol_type="other DNA"
                     /label=synonym:Shuttle Cloning Vector pSET2
                     /note="synonym:Shuttle Cloning Vector pSET2"
                     /db_xref="taxon:125568"
                     /organism="Shuttle vector pSET2"
     gene            156..176
                     /gene="dso"
                     /label=dso
     misc_feature    156..176
                     /gene="dso"
                     /label=double-stranded replication origin
                     /note="double-stranded replication origin"
     gene            367..504
                     /gene="copG"
                     /label=copG
     CDS             367..504
                     /codon_start=1
                     /gene="copG"
                     /product="CopG, transcriptional repressor protein"
                     /function="transcriptional repressor protein"
                     /label=copG
                     /protein_id="BAB03239.1"
                     /translation="MKKRLTITLSESVLENLEKMAKEMGLSKSALISVALENYKKGQVK
                     "
     gene            550..1230
                     /gene="repB"
                     /label=repB
     CDS             550..1230
                     /codon_start=1
                     /gene="repB"
                     /product="RepB, replication initiation¡§Ctermination
                     protein."
                     /function="The plasmid-encoded initiator of replication.The
                     protein introduces a strand- and site-specific nick in the 
                     double-stranded replication origin, on supercoiled DNA."
                     /label=repB
                     /protein_id="BAB03240.1"
                     /translation="MAKEKARYFTFLLYPESIPSDWELKLELLGVPIAVSPLHDRDKSD
                     VEGQQYKKPHYHVIYVSKNPVTADSVRMKIKRSLGDNSVALVQIIRTSIENTYLYLTHE
                     SKDAIEKKKHVYDKADITLLSNFDIDRYITLDVEEKDDMLNEVCDLIDEYDIANMRELR
                     RFIKLHGAEHGLPSIKIINSVLRSHTGLIRLYFDAVYQERKYGSNIPNVNQETGEILSE
                     DSDD"
     gene            complement(1686..2453)
                     /gene="add9"
                     /label=add9
     CDS             complement(1686..2453)
                     /codon_start=1
                     /gene="add9"
                     /product="spectinomycin adenyltransferase, aso known as
                     aad9."
                     /function="spectinomycin resistance"
                     /label=spc
                     /protein_id="BAB03241.1"
                     /translation="MRRIYLNTYEQINKVKKILRKHLKNNLIGTYMFGSGVESGLKPNS
                     DLDFLVVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIELTIIIQQEMVPWNHPPKQ
                     EFIYGEWLQELYEQGYIPQKELNSDLTIMLYQAKRKNKRIYGNYDLEELLPDIPFSDVR
                     RAIMDSSEELIDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERI
                     LLAVRSYLGENIEWTNENVNLTINYLNNRLKKL"
     gene            complement(2855..3178)
                     /gene="lacZ'"
                     /label=lacZ'
     CDS             complement(2855..3178)
                     /codon_start=1
                     /gene="lacZ'"
                     /product="beta-galactosidase alpha fragment"
                     /label=lacZ'
                     /protein_id="BAB03242.1"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     3088..3104
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3105..3161
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(3174..3190)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3198..3214
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3222..3252)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3267..3288
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     gene            4094..4235
                     /gene="ssoA"
                     /label=ssoA
     misc_feature    4094..4235
                     /gene="ssoA"
                     /label=single-stranded replication origin
                     /note="single-stranded replication origin"
ORIGIN
        1 attaattttg aaatattaaa tatttttgga tatataggtg ttaagtttga aattattacc
       61 gcttatgaaa atgggaaatt agttgaaacg caaattattg tggatgacat agagttgata
      121 aaagttgaaa aattttttga ttaaagagaa gaagaggggg cgtactacga cccccctttt
      181 aagtgccgag tgccaaattg ctaaaaaaag tgccgtaaag ctagatacat caagggttta
      241 gctcatgcta cacttcggcg acattttggc gacattttcg gcgacaaaaa gaaaaaaata
      301 cgataaatgt tgcaatgtat gcacagttat gctactatgg ttttataaat ttttgagagg
      361 tggaacatga aaaaaagatt gacgataaca ctcagtgaat cggtgcttga aaatcttgaa
      421 aaaatggcaa aagagatggg gttgtcaaaa tctgcactga tttcagttgc cttggaaaac
      481 tacaagaaag gtcaagtaaa ataaaaaaaa gccgagctgg caggcgggct tttaaaagta
      541 ttataactta tggctaaaga aaaagcaaga tacttcactt ttttactcta tcctgagtca
      601 atccctagtg attgggaatt gaaattagaa ttgctgggcg tacctattgc agtcagccct
      661 ttgcatgata gagacaagag tgatgttgag ggacaacaat ataaaaaacc gcactatcat
      721 gtgatttatg tttcaaaaaa tccagtcacg gcagatagtg tgagaatgaa aattaagagg
      781 tcacttggag ataatagcgt tgctctagtt cagataatta gaactagcat tgagaacacg
      841 tatttatatc ttacgcatga atcaaaggac gctatcgaaa agaaaaagca cgtctatgat
      901 aaggctgaca taactttgct tagtaacttt gatatagacc gttatataac gcttgacgtc
      961 gaagagaaag acgatatgct caatgaggtt tgtgacctta ttgatgagta tgatattgca
     1021 aatatgcgtg aattgagacg ttttattaaa cttcatgggg ctgaacatgg tttacctagt
     1081 atcaaaatta taaattcagt tttacgttcg catactgggt taatacgtct gtattttgat
     1141 gctgtctatc aagaacgtaa gtatggcagt aatatcccta atgttaatca agagacaggc
     1201 gagattttaa gtgaggatag cgatgactga tagaaatgag ctgattaacg atattgcaga
     1261 gttgaaagca aaaagagaca gactactggc tcaaatgaaa gaggcggaac aatgggaaag
     1321 cgtagcgtgg gatagttact atgcagtcgc agaccatgtg aaagctcttg agaaaaagca
     1381 ggagataggt cgaaactatt gggaaagttc tcaaagagct attagtcatc aatttgactt
     1441 tgtggctgac caagctaaca aagtcaaaaa ggtgttggct aaaaagagat atgatttgct
     1501 agatgaggaa atcgacaagc tgatgaacga agttcgagag ctggcagatg tccttgggat
     1561 tgagattgac gagctcggtt tattgttttc taaaatctga ttaccaatta gaatgaatat
     1621 ttcccaaata ttaaataata aaacaaaaaa attaaaaaag tgtttccacc attttttcaa
     1681 tttttttata atttttttaa tctgttattt aaatagttta tagttaaatt tacattttca
     1741 ttagtccatt caatattctc tccaagataa ctacgaactg ctaacaaaat tctctcccta
     1801 tgttctaatg gagaagattc agccactgca tttcccgcaa tatcttttgg tatgatttta
     1861 cccgtgtcca tagttaaaat catacggcat aaagttaata tagagttggt ttcatcatcc
     1921 tgataattat ctattaattc ctctgacgaa tccataatgg ctcttctcac atcagaaaat
     1981 ggaatatcag gtagtaattc ctctaagtca taatttccgt atattctttt attttttcgt
     2041 tttgcttggt aaagcattat agttaaatct gaatttaatt ccttctgagg aatgtatcct
     2101 tgttcataaa gctcttgtaa ccattctcca taaataaatt cttgtttggg aggatgattc
     2161 cacggtacca tttcttgctg aataataatt gttaattcaa tatatcgtaa gttgctttta
     2221 tctcctattt tttttgaaat aggtctaatt ttttgtataa gtatttcttt gctttgatct
     2281 gtcaatggtt cagatacgac gactaaaaag tcaagatcac tatttggttt tagtccactc
     2341 tcaactcctg atccaaacat gtaagtacca ataaggttat tttttaaatg tttccgaagt
     2401 atttttttca ctttattaat ttgttcgtat gtattcaaat atatcctcct cactattttg
     2461 attagtacct attttatatc catagttgtt aattaaataa acttaattta gtttatttat
     2521 agatttcatt ggcttctaaa ttttttatct agataataat tattttagtt aattttattc
     2581 tagattatat atgatatgat ctttcatttc cataaaacta aagtaagtgt aaacctattc
     2641 attgttttaa aaatatctct tgccagtcac gttacgttat tagttatagt tattataaca
     2701 tgtattcacg aacagatctc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     2761 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     2821 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     2881 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     2941 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     3001 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     3061 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattc gagctcggta
     3121 ctcggggatc ctctagagtc gacctgcagg catgcaagct tggcgtaatc atggtcatag
     3181 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     3241 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgctgcgc
     3301 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     3361 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactagaaag
     3421 cccctagaag acgaaataag gactttgaat gaagttctta aggaaaaata cgaggagtta
     3481 gacaaaatcg agctacgagc ctctgagagc ctctcagagc tatctggtgt cgaagggtat
     3541 ataaataacc tagaaaaaca ttcaagcgcc ttagaaagca aaataggggg cttagaaaac
     3601 gaatatttaa gactggctaa acaaaatgcc cgattaagcg acttgaaaat catgtctgaa
     3661 aaagaattgg ctgaaattca gccgaaaaaa ggcatgtttg gaaaagaaca tgtcgaattg
     3721 actaaagagc aatttgagaa atttaaggga ttgatatacc gaagcaagaa tttagttcat
     3781 gaaaaggaat tagagaatga ccaactaagg cggcaaatgc ctctgagaag ctcaaaaaac
     3841 ggttttgagg cgagtttgca acgggctaag gataaaacta aaggagaaag catagaccgc
     3901 cttaaaagcg aaattagagg gcttaaaaat gaaaactcgg ttttgagaca gcaaaatgac
     3961 aaaatgctag gtaagttaag agagtttatg cctgataaag cgttaaataa ttttatttct
     4021 gaacttaaat caataaaacc tatcgtgaag atagttaaaa gagttattga aaaaggctta
     4081 gggctctaag cgatttatgc cgagaaaact cttgctagga agctatgcga aatagactag
     4141 taggggtcga gtcgacaggc tgaaagcttg ccgaccgaac acgacagtca gatttcagca
     4201 taggtttctt cctagcaaga ggaaattgga ataaagaaag cgtatcaaag taaaactaaa
     4261 aggactaagt cctgtgtaat acaggcctta gtcctttact taaaatagaa tgtccaactt
     4321 tttggggtca gtatatattt tagtcttttt tattctatag ttgggataga gatttgtact
     4381 cctcctgcgc catatgcaat tccgcttata cctgcatttc ttgctttttt aggtaaaccg
     4441 aatgaaccac taactgattg acctcctttg ccagatttgc tgacaggata tattctagta
     4501 gtgttatctg agaaagtaac tgttatcaat ccagaatatg tataactttt tgatgtatta
     4561 ggtttaactg accaccaaac ttctcgacca ccaccatata attcgatttt tgtatatccg
     4621 ccatttccgt aacttgtttt gtaaggaact atttcaccgg tataagaata atcagttgta
     4681 gttgaaatat ttgtatttga ggtagtgact gtattatctg caaaaacagt agaaatggat
     4741 ataaatccta acatagaaaa aagagtgaca aatacaatta ttgatttttt gaaacttttc
     4801 atgaaagttt acctccgttt ttttgttata attatagcaa aaaaaataat taaaaagaaa
     4861 gcgttatcat agaaatgaaa aagaatttta ttgcaattat aaaaaacata ttaaaaaaca
     4921 aaaatatccc taaatattgt tttgaaatta ttgaaaaaga tggaagtact tgttttcttt
     4981 cgacaaatta ctttgatgta
//
